Cell Culture

Cell Culture

Gas exchange of cell culture medium (oxygen supply, CO2 removal, PH adjustment)

  Cell culture is the process of taking cells from animals or plants and growing them in a favorable artificial environment. Culture conditions vary widely from cell type to cell type, but the artificial environment in which cells are cultured always consists of an appropriate container containing the following:

  • Substrate or medium that provides essential nutrients
  • Growth factors
  • Hormones Gases (O2, CO2)
  • A controlled physicochemical environment (pH, osmotic pressure, temperature)

  Among these, the supply of oxygen to the culture medium and the failure to control the CO2 generated and adjust it to a pH zone suitable for the culture of those cells will affect the various metabolic pathways involved in cell proliferation and product quality. High concentrations of CO2 can also inhibit the growth of animal cell cultures and have a negative effect on their products.

  NAGASEP is a gas separation membrane made of silicone membrane material with the following features

  • Good gas (O2, CO2) permeability and minimal gas loss.
  • Large membrane area due to the use of ultra-thin hollow fiber membranes.
  • Homogeneous membrane prevents contamination by bacteria and other contaminants.
  • Oxygen can be supplied without air bubbles regardless of supply pressure.
  • High heat resistance allows autoclave sterilization.

  NAGASEP is used as a gas exchanger at the heart of culture equipment for pharmaceutical production by taking advantage of these features.